Regulation of glutaminase levels in Escherichia coli

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Regulation of glutaminase levels in Escherichia coli.

Nitrogenous metabolites, cyclic adenosine 3':5'-monophosphate (cAMP), and the stage of culture growth all influence the levels of glutaminase A in Escherichia coli, but no variables in culture conditions alter the levels of glutaminase B. Growth of E. coli on culture media containing glucose and excess ammonia results in a rise in the level of glutaminase A as the cultures enter stationary phas...

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In Silico Analysis of Glutaminase from Different Species of Escherichia and Bacillus

Background: Glutaminase (EC 3.5.1.2) catalyzes the hydrolytic degradation of L-glutamine to L-glutamic acid and has been introduced for cancer therapy in recent years. The present study was an in silico analysis of glutaminase to further elucidate its structure and physicochemical properties.Methods: Forty glutaminase protein sequences from different species of Escherichia and Bacillus obtained...

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On the regulation of guanosine tetraphosphate levels in stringent and relaxed strains of Escherichia coli.

We have examined the intracehular levels of the guanosine tetraphosphate, guanosine 5’-diphosphate 2’or 3’-diphosphate (ppGpp), in an isogenic pair of stringent and relaxed strains of Escherichia coli. As previously observed, relaxed strains do not accumulate ppGpp during amino acid deprivation, while stringent strains do. However, under all other culture conditions examined, relaxed and string...

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ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 1975

ISSN: 0021-9193,1098-5530

DOI: 10.1128/jb.123.3.992-999.1975